Gabriela Borja

3 Questions 3 Answers 0 Followers

Questions related from Gabriela Borja

Have you had problems with primers from IDT?

My lab uses the primers from IDT but since I arrived here I noticed that when the primers arrived they work perfectly but then I started to notice that the bands in the gel become faint and after...

20 June 2025 394 0 View

How can I overcome this problems with the PCR?

Two weeks ago, I run some PCRs, sometimes I cannot get amplification but other times I indeed get it. But now that I am trying to replicate it is not possible, that could not be unspecific bands...

22 May 2025 3,527 3 View

Do you have any recommendation to increase the yield and quality of RNA after immunoprecipitation?

I am trying to isolate RNA from stress granules, these were obtained using immunoprecipitation, but the amount of RNA and sometimes the quality is not good enough.

29 January 2025 2,110 5 View