Gabriel Mello

6 Questions 9 Answers 0 Followers

Questions related from Gabriel Mello

I can't form spheroids from HT-29 cell line. Does anybody know what is happening?

I have been working with 3d spheroids for a few months. I started with HCT-116 colon cancer cell line and have not had any problems, this cell forms tightly aggregated spheroids. However, I have...

12 December 2016 1,813 4 View

Are these true spheroids?

Hello everyone. I'm trying to form spheroids fom hct116 cells. The first time I did the experiment I could see a defined edge around the spheres and they looked tight. Now all I get are those...

03 March 2016 1,727 3 View

How can I determine a clinically relevant concentration?

Hi! I am testing a new compound on colorectal cancer cell lines and I want to compare it's efficacy with the standard drug utilized in the treatment, which is the 5-FU. What concentrations should...

12 December 2015 2,821 5 View

Could someone help me with a problem with western blot?

We are currently facing a problem regarding the Western blot technique. Proteins above 70 kda don't seem to be transferred to the membrane. So far we have ruled out: The system, the samples, all...

10 October 2015 9,703 4 View

How do I work with different cell lines at the same time?

Hi. I am currently working with 3 non-related cell lines. There is a rule in my lab that says we can't work with different cell lines at the same time in the flow hood to avoid...

09 September 2015 7,292 16 View

Cells treated with DMSO show less viability than cells treated with the compound, how is that possible?

I have performed a MTT viability assay to see how my cells (MCF-7 and HCT-116) respond to a drug. I had one control with cells + medium and another control with cells + medium + DMSO in different...

08 August 2015 9,103 15 View