Gabriel Gama

2 Questions 6 Answers 0 Followers

Questions related from Gabriel Gama

What could negative 230 and 280 nm mean in nanodrop one?

We have done some RNA seq libraries and quantified the final product in nanodrop. As always, we blanked with the used solvent (0.1 TE buffer). Most samples are really good, around 6-20ng total...

3 weeks ago 4,188 3 View

Q5 Site-Directed Mutagenesis Kit primers being inserted in my plasmid, how to solve?

Hello all, I'm trying to do minigene assays for multiple genes. I got 5 genes to work for the wildtype using pET01 exon trap vector, and the splicing assay worked fine. Now I'm trying to...

04 August 2025 6,502 2 View