Gabby Budziszewski

3 Questions 4 Answers 0 Followers

Questions related from Gabby Budziszewski

Slow-to-crystallize model protein?

Hi all! Is anyone aware of a model protein that crystallizes, albeit slowly? (~3-6 weeks)

25 October 2022 6,894 4 View

How to use XDS to merge data in lower symmetry?

I have a dataset from x-ray crystallography that is has poor statistics from processing (high Rmerge) and does not refine particularly well. I ran the merged reflections through Xtriage (Phenix)...

15 December 2021 1,780 2 View

Is there a problem with trying to make a recombinant baculovirus using a pFastbac construct that has a bacterial IRES preceding the gene?

With the bacterial vectors I am using, convenient cloning sites would insert the gene and also a 5' bacterial IRES into pFastbac1. Will this cause problems downstream in trying to make a...

05 April 2018 867 4 View