What is the relationship between amount of cysteines in a recombinant protein vs glutathione concentration for re-folding?

I need re-fold a recombinant protein that has more than 50 Cysteine residues, I know that usually we use the ratio 1:5 or 1:10 of Glutathione reduced and oxidized in order to re-fold recombinant...

02 July 2020 5,908 0 View