Frederik Link

4 Questions 6 Answers 0 Followers

Questions related from Frederik Link

How to break down protein crystal agglomerates?

Dear all, I would like to know how to break down protein crystal agglomerates. My crystallisation experiments yield quite a high number of crystal agglomerates (see images attached for 10x and...

04 July 2022 9,018 3 View

Lysozyme solution for crystallisation turns cloudy immediately after mixing lysozyme with NaCl , however it worked before, why?

Hi there, I am carrying out crystallisation experiments for lysozyme. The method is the following: dissolve 73.6 mg/ml lysozyme (lyophilised or crystalline powder) in 0.1 M sodium acetate buffer,...

13 December 2021 8,704 8 View

Do free cysteine molecules interact with disulfide bonds in proteins?

Hi all! I would like to study the interaction between free L-Cysteine and disulfide-bonds in proteins (e.g. whether the disulfide-bond between proteins gets "broken" and a new disulfide-bond...

05 May 2021 3,677 3 View

Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

07 February 2021 9,530 7 View