Frederic N Daussin

4 Questions 5 Answers 0 Followers

Questions related from Frederic N Daussin

Why no NAD and only NADH content in skeletal muscle of mice using commercial kit ?

W tried to determine the NADtotal and NADH on skeletal muscle of mice (gastrocnemius). Unfortunately, we obtain the same values for NADtotal and NADH that suggest we do not have NAD in our sample...

05 May 2018 3,912 1 View

Is Carboxymethylcellulose stable over few day ?

Does anyone knows if carboxymethylcellulose is stable during 10 days. We should provide a drug to our animals and we will use CMC as a vehicle but the dose of drug avec very low so I'm wondering...

03 March 2018 5,366 3 View

Why do you use the log-transformed value to express western blot results?

The study of Coffey et al. (2009) compares the effect of diverse contractile activity in human skeletal muscle. They measured phosphorylation of several proteins and the results (phospho/total)...

09 September 2014 4,010 3 View

Is it possible to use protein extraction with RIPA buffer to measure kinase activity by ELISA?

We have human samples. We have used RIPA buffer to extract protein and to perform western-blot analysis. I'm interested to measure AMPK activity in these samples but I don't have froze muscle.

07 July 2014 2,897 5 View