Frank Crittenden

3 Questions 1 Answers 0 Followers

Questions related from Frank Crittenden

When should I add antibiotics to cell culture media?

To be clear: I'm asking about pen/strep to prevent contamination, NOT selective antibiotics. Should I add the antibiotics when I am making the media? Or should I add the antibiotics directly to...

11 November 2019 8,077 3 View

When/how should I select cells with puromycin after plasmid delivery?

I am delivering the pSpCas9(BB)-2A-puro plasmid to SH-SY5Y cells via electroporation, and I am trying to come up with a protocol for the puromycin selection. All of the published literature I can...

06 June 2019 5,246 4 View

Plasmid transfection in SH-SY5Y cells: best reagent?

I want to transfect a 9.2 kb plasmid into SH-SY5Y cells, but my university does not have an electroporation system. What transfection reagent works best for transfecting these cells?

02 February 2019 3,342 3 View