Francisco Navarro

4 Questions 14 Answers 0 Followers

Questions related from Francisco Navarro

Why do my SDS-PAGE gels have problems to polymerize?

For some months we have had problems with SDS-PAGE polymerarization. We have changed the acrylamide/bisacrylamide solution, the TEMED, the APS but the problem continues. Does anyone have an...

05 May 2013 7,020 6 View

TCA protein precipitation and PEG?

I have my purified proteins in a solution containing 37% PEG 8000. I tried to precipitate the proteins by using TCA but it doesn't work. Does anyone know how to concentrate or precipitate my...

05 May 2013 5,934 4 View

What is the best method to measure DNA concentration, after ChIp, to perform library construction for ChiP-seq?

We have observed that using qubit, nanodrop or Byonalyzer data are very different.

04 April 2013 9,319 1 View

Has anyone performed Chip-seq from s.cerevisiae in the Ion Torrent Machine? Some considerations?

Some considerations?

04 April 2013 9,773 2 View