Francisca Villavicencio Tejo

3 Questions 5 Answers 0 Followers

Questions related from Francisca Villavicencio Tejo

¿What is the best protocol to obtain isolated- purified mitochondrial fraction from mice brain?

We are currently performing this protocol in tissue obtained from mice (Cortex and hippocampus) following the next steps: MSH Buffer : 230 mM Manitol 70 mM Sucrose 5 mM HEPES pH: 7,4 +protease...

11 May 2022 6,482 1 View

¿What is the best disolution to use Sulforaphane ( liquid insoluble in water, soluble in DMSO) for i.p treatment in mice?

i've read a recomendation of 17% DMSO + saline solution

01 July 2021 1,567 0 View

What is the best technique for differentiating 3T3 L1?

the standard protocol (12K DMEM + 10% FBS + 100 mM + 1 mM IBMX + 10ul insulin DEXA) has been slow to get results, it's been eight days and still are as fibroblasts.

26 November 2015 5,017 6 View