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Questions related from Francesco Perini
The PCR of my samples look great. I used 0.5 uL of TruI enzyme and 2 uL of 10X buffer for digestion (1h @ 65°C) of PCR (432 bp). Then I used 5 uL of dye for 22.5 uL of digested PCR, and run 12 uL...
02 February 2021 9,813 3 View
This is an agarose gel run of my rna samples extracted from liver and ladder. I have to carry out reverse transcription in cDNA and then do a real time PCR. Are they degraded? Will real time work?
15 May 2020 5,744 0 View
I have already done a RT-PCR with RNA from rabbit testis. I used Beta-2 microglobulin as housekeeping gene. Some problems were occured because the expression levels were very different among...
02 October 2019 1,298 3 View
