Francesco Antonio Tucci

4 Questions 3 Answers 0 Followers

Questions related from Francesco Antonio Tucci

Can I pool WT and CRISPR/Cas9 KOs cells in the same scRNAseq sample and demultiplex them by puromycin resistance cassette expression?

Library preparation for single cells RNA sequencing ends being the most expensive part of the of the process, so it's way cheaper doubling the reads depth than the number of samples. I have seed...

21 October 2020 831 0 View

RNAseq variant calling: why STAR align promotes mismatched overhanging mappings onto introns instead of calling splicing junctions?

Hi, I'm doing variant calling from my RNAseq data on a cell line (Paired End 2X100b, Illumina) I'm following GATK workflows and everything is working almost fine since I can find all the expected...

07 October 2020 4,368 0 View

Why do I get an error in getBamCounts when adding a fasta reference (ExomeDepth)[solved]?

Whenever I try to add a reference fasta file for computing the GC content in the GetBamCounts function: my.countsV6

12 May 2020 5,308 5 View

How I make sense out of Whole Exome Sequencing on cancer cell lines?

I hope you can give me some insights on the right direction to pursue and on some bioinformatic tools I'm ignoring to solve the following bioinformatic problem: I got involved in a cancer research...

02 May 2020 5,995 0 View