Francesca Ceroni

7 Questions 10 Answers 0 Followers

Questions related from Francesca Ceroni

What is a suitable Minimal Medium for mammalian cell cultures?

Hi all, I am looking for an equivalent of the M9 cuture medium in bacteria but for mammalian cells. I would need to grow my cells in very controlled conditions [sugars, aa, vitamins...etc]. Does...

05 May 2015 2,944 4 View

Qubit vs. Nanodrop?

Can someone tell me if Qubit is really better than Nanodrop in nucleic acids quantification, even for small concentrations?

01 January 2014 9,649 39 View

ds cDNA synthesis for RNAseq?

I am about to perform RNAseq on E. coli total transcriptome. I am planning to use the Nextera kit so I need to prepare my own cDNA from total RNA. Since the kits for ds cDNA synthesis are crazily...

01 January 2014 7,590 14 View

Do you know the protocol for optimizing fragment size with random primers?

Does anybody have or know a protocol to understand how to optimize RNA/random hexames/temperature in ds cDNA synthesis reaction to get a precise fragment size?

01 January 2014 6,195 2 View

Does anybody know which is the size range of fragments yielded by a first strand cDNA synthesis reaction starting from RNA?

Is there any way to control it?

01 January 2014 7,285 6 View

Single read vs paired end sequencing in RNAseq?

Which is the advantage of performing a paired end sequencing instead of a single read one? I know it gives information for eukaryotic samples about the splicing variants... but for prokaryotes?

01 January 2014 9,505 6 View

Expression of beta hCG in Escherichia coli - can anyone help?

I am trying to express Beta hCG in Escherichia coli but I can`t see any 23 KDa band on my SDS page. I know it forms inclusion bodies, but what I am using is codon optimized. I am wondering if this...

11 November 2013 855 5 View