Has anyone observed the appearance of a double positive CD4/CD8 population after Dynabeads stimulation?

I am using PBMC from normal donors, I have non-stimulated controls and 24hr stimulated samples with 1:1 Dynabeads. In the non stimulated samples, my CD4 CD8 gating looks normal (only 2 pop: either...

25 February 2020 7,926 2 View