Francesca Bertino

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Questions related from Francesca Bertino

Lipofectamine 3000 in 10 cm dish?

Dear all, I'm trying to transfect HeLa cells with lipofectamine 3000 in a 10 cm dish. Do someone of you have a protocol to share? I still examined the table for scaling up or down lipo3000 but it...

14 October 2020 3,254 3 View

Can the pcDNA5FRT/TO vector be used for transient overexpression of a gene in a common cell line?

Hi,I have my gene of interest in pcDNA5/FRT/TO vector. Can the pcDNA5FRT/TO vector be used for transient expression of the gene in a common cell line (different from the Flp-In™ T-REx™ System)?...

02 March 2020 3,337 2 View

Does the GFP protein correctly fold and emit fluorescence inside the mitochondria?

I would like to add the GFP tag to the C-terminus of my protein, the protein is located on the membrane of mitochondria and its C-terminus is located inside the mitochondria. Is it possible that...

06 March 2019 7,949 2 View