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I performed a transient transfection of premiR , I have analyzed the efficiency of transfection by flow cytometry after 24 , 48 and 72 hours after transfection through the analysis of cells...
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I did a western blot to detect MET of 145 KDa in cell trasfected with premiR of miR1, or premiR of miR133b, or in cell untrasfected or in cell trasfected with premiR scramble, I loaded the samples...
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