Forest Ray

3 Questions 11 Answers 0 Followers

Questions related from Forest Ray

Can dam methylation sites become methylated in eukaryotic cells?

I inadvertently created a dam methylation site in some lentiviral constructs that I intend to transduce SNB19 cells with. If I amplify these plasmids in dam-/dcm- cells, is there a risk that the...

03 March 2013 7,790 2 View

How do I design primers to amplify highly structured non-coding regions of genomic DNA?

I've been trying to clone a set of human promoters and have been having very mixed success. The problem I'm having is that I frequently amplify unexpected sequences from genomic DNA using primers...

12 December 2012 7,141 17 View

How to amplify a specific region of genomic DNA via PCR?

I want to amplify twelve1kb-long promoter regions from genomic DNA for some future experiments. The primers that I have designed to do so are all 19-28 nucleotides in length, with similar melting...

09 September 2012 9,043 84 View