Forest Ray

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Questions related from Forest Ray

Can dam methylation sites become methylated in eukaryotic cells?

I inadvertently created a dam methylation site in some lentiviral constructs that I intend to transduce SNB19 cells with. If I amplify these plasmids in dam-/dcm- cells, is there a risk that the...

08 March 2013 4,437 2 View

How do I design primers to amplify highly structured non-coding regions of genomic DNA?

I've been trying to clone a set of human promoters and have been having very mixed success. The problem I'm having is that I frequently amplify unexpected sequences from genomic DNA using primers...

11 December 2012 2,070 15 View