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Hello everyone, I am performing IHC for von Willebrand Factor on femurs and tibiae of 22-week-old SD rats. The bones are decalcified in EDTA and paraffin embedded. After antigen retrieval,...
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Hello everyone, I have fixed 22-week-old rat femurs in 10% formalin for 24hrs, then decalcified in 10% EDTA for 28 days. I have read a lot recently that over-fixation is not too much of an...
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