Floyd Mattie

2 Questions 8 Answers 0 Followers

Questions related from Floyd Mattie

How long can I store conditioned medium?

I am looking to stockpile some conditioned medium prior to starting a clonal isolation of HepG2 cells. It has been my previous experience that most HepG2 cells sorted as individual cells into...

25 June 2016 1,403 1 View

Seeking advice on transfecting ARPE-19 cells. Does anyone here have experience transfecting ARPE-19 cells?

I am currently trying to transfect plasmids for expression of fusion proteins into ARPE-19 cells, and the Lipofectamine 2000 is causing a distinct morphological change. With or without DNA, the...

07 May 2015 1,040 14 View