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Questions related from Florian L Muller
Cyclophosphamide is supposed to be activated by Cytochrome P450. I've treated Huh7 and PLC liver cancer cell lines with Cytochrome P450, but there is no sensitivity whatsoever, even at 500uM....
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I have an Emmons-Horner-like agent (triethylphosphonoacetate) that I would like to label with tritium. The alpha proton is readily exchangable in NaHCO3/D2O; I presume the same would work with...
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I prepared a 50 mM phosphate buffer in H2O and pH'ed it to 7. I lyophilized it and dissolve it in D2O? What is the pD? And what would be the pT (I would like a tritium water solution, ultimately).
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Many enzymes have preference for L versus D or R versus entiomers. Is there a precendent in the literature for changing, engineering by amino acid alteration, a D-selective into an L-selective enzyme?
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I need to measure RFP (red fluorescent protein) induction in FFPE sections. I know RFP is expressed by fluorescence when I dissect the brain, I just need to make some pretty-looking slides.
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I have xenografted human tumor lysates that I am running western blots on. I need to determine the extent of mouse cell contamination in these lysates (I don't have slides for the tumors). I am...
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