Florencia Malizia

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Questions related from Florencia Malizia

Someone with experience with alpha-synuclein aggregation?

Hello everyone! I am interested in generating alpha-synculein oligomers and fibrils from monomeric alpha-synuclein and then calculating the percentage of aggregation. I have this protocol: I...

12 January 2021 7,453 3 View

Native-PAGE. Could anyone have a protocol or some advice to give me?

Hello everyone. I want to do a Native-PAGE followed by a western blot analysis to detect the different native forms of my proteins of interest (synuclein family). Could anyone have a protocol or...

03 January 2021 5,824 4 View

Has anyone measure melanin content in in vitro cell culture?

I work with B16-F10 and SK-MEL28 melanoma cells and I want to measure extracellular and intracellular melanin but I found different protocols and I have some questions. To measure extracellular...

29 November 2020 1,356 3 View