Flávia Moreira da Fonseca

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Questions related from Flávia Moreira da Fonseca

Why does a Nested PCR protocol amplify samples and not amplify positive control?

I have this problem. Today I used a new positive control of an isolated NC-1 strain in cell culture and the problem persists. I performed a serial dilution test to test the sensitivity of the...

09 December 2020 6,422 2 View

Is the pipetting order of the positive and negative controls in the PCR important to avoid in a possible contamination?

I have been watching bands form on my negative controls. I have already changed all reagents, optimized the hygiene of materials and the environment and increased care in pipetting.

08 December 2020 9,204 3 View

How to avoid the formation of traces and dimers in DNA gel electrophoresis?

My protocol Primary PCR (NP6/21): Initial Denaturation at 94C, 2 min.; 30 cycles Denaturation 94C 30 sec, Annealing 65C 1 min., Extension 72C 1 min.; Final Extension 72C 5 min.; Nested PCR...

08 December 2020 3,259 3 View

How to standardize and obtain a good PCR reaction with samples with a low and variable amount of DNA?

I work with the detection of N. caninum DNA in milk samples in milk samples. The DNA concentrations in my samples range between 2-90 ng / ul. I would like to establish and standardize a final...

02 December 2020 8,907 3 View