What are the most suitable conditions for analysing acetic acid release during enzyme assay using reverse phase HPLC column?

Column = Thermoscientific hypersil gold column Mobile phase = 0.005M H2SO4 (pH 2.1), Temperature = 45oC, Flow rate = 0.6ml/min Detector = refractometer. Acetic acid standards concentrations...

11 November 2015 2,325 3 View