Fiyin Adenekan

2 Questions 1 Answers 0 Followers

Questions related from Fiyin Adenekan

Can i excise the bands from my single digest or repeat digest again ?

I digested 5ug of pLentiv2 (12kb) with 1uL BamHI-HF (NEB) at 37C for 2 hours in a 20uL volume with no heat inactivation required. I ran the digested vector on a gel (lane 4), and detected the...

09 July 2022 4,902 2 View

Is it ok that the cut vector travels the same length as the uncut vector on a gel ?

I carried out digestion of vector pLentiv2 (14kb) with restriction enzyme BsmBI at 37C for 3 hours, and performed a gel extraction to purify the cut DNA fragment for cloning. I ran both the uncut...

22 August 2021 3,476 1 View