Fitria Zahra Oktavia

5 Questions 7 Answers 0 Followers

Questions related from Fitria Zahra Oktavia

Why my forward primer found in reverse sequencing result, instead on forward sequencing result?

I sequenced my amplicon using Sanger sequencing method, and received the two .Ab1 file which is the forward and reverse sequencing result. However, when I checked my primer binding in those...

22 November 2018 5,815 5 View

Why my forward primer found in reverse sequencing result, instead on forward sequencing result?

I sequenced my amplicon using Sanger sequencing method, and received the two .Ab1 file which is the forward and reverse sequencing result. However, when I checked my primer binding in those...

11 November 2018 7,331 5 View

Help in cDNA synthesis, please?

I am working with unknown sequence gene in a plant, and I just found the partial gene of its actin gene. So I use my actin primer for check my cDNA quality. But from some cDNA stocks, I just get...

12 August 2018 2,059 2 View

What if PCR product from cDNA is greater that PCR product from DNA? Is it possible?

I need do gradient temperature for knowing the exact annealing temperature of my actin primer. So, I do amplification both on DNA and cDNA. But I got the band are larger in cDNA than in DNA,...

08 July 2018 6,708 5 View

What if PCR product from cDNA is greater that PCR product from DNA? Is it possible?

I need do gradient temperature for knowing the exact annealing temperature of my actin primer. So, I do amplification both on DNA and cDNA. But I got the band are larger in cDNA than in DNA,...

07 July 2018 7,449 6 View