Fitria Zahra Oktavia

4 Questions 6 Answers 0 Followers

Questions related from Fitria Zahra Oktavia

Why my forward primer found in reverse sequencing result, instead on forward sequencing result?

I sequenced my amplicon using Sanger sequencing method, and received the two .Ab1 file which is the forward and reverse sequencing result. However, when I checked my primer binding in those...

22 November 2018 5,788 5 View

Why my forward primer found in reverse sequencing result, instead on forward sequencing result?

I sequenced my amplicon using Sanger sequencing method, and received the two .Ab1 file which is the forward and reverse sequencing result. However, when I checked my primer binding in those...

11 November 2018 7,305 5 View

What if PCR product from cDNA is greater that PCR product from DNA? Is it possible?

I need do gradient temperature for knowing the exact annealing temperature of my actin primer. So, I do amplification both on DNA and cDNA. But I got the band are larger in cDNA than in DNA,...

08 July 2018 6,686 5 View

What if PCR product from cDNA is greater that PCR product from DNA? Is it possible?

I need do gradient temperature for knowing the exact annealing temperature of my actin primer. So, I do amplification both on DNA and cDNA. But I got the band are larger in cDNA than in DNA,...

07 July 2018 7,428 6 View