Firdos Firdos

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Questions related from Firdos Firdos

Can someone provide me with a protocol for freezing and reviving pancreatic beta cells (MIN6)?

I am trying to revive my cells, but they have not attached to the surface. For freezing, I use 10% DMSO, 20% FBS, and 70% DMEM (freezing media composition). When I stored them, their passage...

13 April 2018 9,592 2 View

Can someone provide me with a protocol for freezing and reviving pancreatic beta cells (MIN6)?

I am trying to revive my cells, but they have not attached to the surface. For freezing, I use 10% DMSO, 20% FBS, and 70% DMEM (freezing media composition). When I stored them, their passage...

13 April 2018 3,633 2 View