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Questions related from Fiorella briceño nuñez
Hi, I'm trying to amplified and get the sequence of one gene (4kb) in 10 sorghum accessions and I divided in 3 fragments the gene (I fragment: 1.3kb, II fragment: 2.7kb and III fragment: 0.8 kb)....
04 April 2017 2,871 6 View
Hi! I could amplified one gene (4kb), but it was divided in 3 fragments (size: 2.9kb, 2.7kb and 0.8). When I tried to alignment this 3 fragments to get the complete structure, they told me that is...
09 August 2016 5,052 2 View
I am having trouble with cloning. My PCR product is 1kb. After ligation (with pGEM easy vector) into the plasmid, I transformed into JM109 competent cell. I can get white colonies but when I did...
18 February 2016 6,536 3 View