Fiona Wahinya

2 Questions 9 Answers 0 Followers

Questions related from Fiona Wahinya

Does anyone have experience with RNA isolation from dead plant tissues like roots?

I have tried isolating RNA from dead roots with no success. We call them dead just because they are discolored and black/dark brown but not sure if that means they are completely dead. I have...

01 February 2024 5,301 4 View

My colony PCR and sequencing results do not match. What could be the problem?

I have recently started cloning a gene of 2.6Kb in TOPO-Zero blunt vector. I amplified my gene with KOD FX Neo and got the correct size band, then proceeded to clone into TOPO blunt vector. After...

01 January 1970 2,023 15 View