Fiona Brown-Burke

3 Questions 3 Answers 0 Followers

Questions related from Fiona Brown-Burke

Why would ponceau and B actin show signal but H3 does not on a Western Blot?

I have a western blot mystery. I see protein on my ponceau stain, my beta actin is relatively even, but two of my histone three lanes are basically missing signal... This is not the first time...

13 February 2025 5,043 5 View

Besides 10x, are high/low PCR magnets available anywhere else?

After working with the High/Low magnet from 10x in our core facility, I found it super useful for DNA clean up and would love to use it for other protocols in my lab. Does anyone know if you can...

10 July 2024 5,609 0 View

Shrinking cells after FOXP3 Perm/Fix for Flow cytometry normal?

I've been starting to use the eBioscience FOXP3 fix/perm kit and have noticed that my cells shrink to almost in half (FSC). Has anyone else noticed this? Any suggestions for how to prevent this?...

09 November 2022 5,285 3 View