Finny Varghese

2 Questions 7 Answers 0 Followers

Questions related from Finny Varghese

Can anyone suggest a reliable protocol for fixation and immunostaining of C6/36 cells infected with Chikungunya virus?

Infecting C6/36 cells with high MOI of CHIKV. Fixed using 4% PFA and followed two different protocols for immunostaining - one which had an extra blocking step using PBS containing 5% FBS as well...

14 May 2014 5,611 6 View

Can anyone help with problems with MIDI prep yields of a high copy number plasmid?

I am trying to amplify an infectious cDNA clone of a plus-stranded RNA virus. The clone is full-length genomic sequence of a plus-stranded RNA virus, under the SP6 promoter in a high copy number...

27 November 2013 9,905 2 View