Fidel Ovidio Castro

2 Questions 2 Answers 0 Followers

Questions related from Fidel Ovidio Castro

Dot/Slot blot for proteins

Hi there, I need to perform dot or slot blot for proteins, but I do not have a manifold device. I recall when I was "younger" people use to dry out membranes without vacuum. Any suggestion?

07 July 2017 5,012 0 View

Hi there, I need to quantify cAMP variations in a cell line

Hi, I need to quantify the levels of cAMP intracellularly in a cell line after stimulation of the EP2 receptor with PGE2. In the web I found severa protocols, most of them using radioactivity,...

01 January 1970 5,204 4 View