Fernando Tessaro

4 Questions 11 Answers 0 Followers

Questions related from Fernando Tessaro

Does anyone has tried to use GAPDH expression in LPS stimulated macrophages?

Hi everyone. I would like to know if someone have already used anti-GAPDH (G5259) to western blot. I tried once diluted 1:10000 in 5% low fat dried milk/TBS-T for 1 hour in RT. The same way I use...

03 March 2016 484 2 View

Is 30 minutes enough time to secrete pro inflamamatory cytokines of macrophages stimulated by K. pneumoniae?

Hi ! Anyone measured cytokine secretion (IL-1beta and TNF-alpha) from macrophages stimulated by K. pneumoniae? I want to determine in cell culture of killing assay (30 minutes; MOI: 50)? Is time...

11 November 2014 2,595 14 View

Can anyone suggest how to perform a killing assay using peritoneal macrophages from peritonitis caused by injection of S. aureus?

Hi everyone! I want to perform a killing assay using peritoneal macrophages from peritonitis caused by a injection of S. aureus. Does anyone know how to perform it, using a plate 96-well and...

10 October 2014 8,228 3 View

Isolation of alveolar macrophages

Hi everyone! How many cells do you used to collect by bronchoalveolar lavage from male C57BL/6 mouse? I would like do more assays but my cell count is low. Thank you!

09 September 2014 2,798 8 View