Fernanda Rufino da Silva

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Questions related from Fernanda Rufino da Silva

Why do only 1:10 diluted cDNA samples amplify consistently in qPCR, while undiluted samples fail, despite multiple optimizations and proper controls?

During the gene expression analysis of the lipases LIP1 and LIP2, as well as the reference gene β-tubulin, using cDNA obtained from RNA extracted with TRIzol, synthesized with the High Capacity...

last month 1,481 4 View

How to Identify and Eliminate Contamination in qPCR Negative Controls?

I am conducting an experiment on comparative gene expression in yeasts using the real-time qPCR method. However, I am experiencing amplification in all my negative controls. I use MilliQ water,...

21 November 2024 9,326 4 View