Fernanda Amorim

3 Questions 8 Answers 0 Followers

Questions related from Fernanda Amorim

How can I disaggregate the proteins of Pichia supernatant without denaturing agents?

I need purify a recombinant protein expressed by the Pichia pastoris system. However, it doesn't have His tag and seems that it is aggregating with the Pichia components...Furthermore, I can't use...

29 June 2014 486 8 View

Why my colony PCR is positive but when the clone is sequenced there isn't an insert?

I am trying to cloning a protein in the pPICZalfaA vector, with the restriction enzyme SalI and XhoI, the colony PCR (with the specific forward primer for insert and reverse primer of vector) is...

17 February 2014 8,091 13 View

How can I purify proteins from the SDS-PAGE gel without blotting? Can I use this protein to do Edman's degradation?

Is there any protocol or kit to do this?

23 November 2012 486 13 View