Fergal Waldron

2 Questions 2 Answers 0 Followers

Questions related from Fergal Waldron

What are typical recovery rates of RNA from an RNAClean XP purification; with high levels (ugs) of Trizol extracted input RNA? 15% typical?

After RNAClean XP purification I recover on average 15% of input RNA (3 ug - Trizol extracted). Just wondering if this was typical?

06 October 2020 3,714 2 View

Can anyone recommend a PCR primer design tool which can find the largest ORF and allows you to specify amplicon size/range?

I'm looking for a PCR design tool which not only identifies the largest ORF from a DNA sequence, but crucially can design primers for a desired amplicon size/range within that ORF. Preferably...

10 June 2016 4,197 3 View