Has anybody transfected an integrative plasmid DNA into rat bone marrow-derived mesenchymal stem cells by lipofectamine 2000 with a high efficiency?

I am working with rat BM-MSCs, and i am trying to transfect these cell with integrative pIRES-EGFP plasmid by applying lipofectamine 2000 (invitrogen). however I found these cells very hard to...

01 September 2018 9,979 3 View