Is possible that primers GPO3-MGSO used for mycoplasma detection could amplify a double band?

Hi everyone, I'm doing PCR for mycoplasma detection I'm using the primers GPO-3 and MGSO; the denaturation, annealing, and elongation temperatures and times were 95oC for 2min, 95oC for 30s, 57oC...

25 March 2024 9,917 4 View