Felix Toka

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Questions related from Felix Toka

Does anyone know where I can purchase or get the BoMac cell line?

I have noticed that many papers have used the BoMac cell line (Bovine macrophage) to do a number of things. However, none happen to mention where they got those cells. I would like to test mRNA...

28 October 2015 6,209 6 View

Can a CMV promoter drive expression of a foreign non-secreted protein by a poxvirus?

I am trying to construct a recombinant poxvirus expressing a fluorescent protein, to be used as an indicator system. I do not have access to a vector with a homologous poxvirus promoter and so I...

06 March 2014 3,504 8 View

English language usage: gene expression or gene transcription in describing RT-PCR results?

Gene expression has two main processes; DNA to RNA called gene transcription, followed by RNA to protein called translation. When we perfom RT-PCR we use mRNA to look for genetic information...

05 March 2013 8,214 36 View

Does anyone have experience with ELISA kits for measuring canine cytokines?

I want to measure IL-1beta, IL-4 and IL-12 produced by canine leukocytes by means of ELISA. Intracellular staining (FACS) appears not to be working well. I would appreciate suggestions on a...

26 September 2012 4,192 3 View