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I often use a speed vacuum concentrator to concentrate my DNA prior to restriction digests, DNA ligations, NGS library preparation or target captures. Usually, my DNA is eluted in 10 mM Tris-Cl...
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We are trying to immunolabel a transcription factor in 50 µm thick mouse retina vibratome sections (embedded in agarose). Even after the 12th repetition of the experiment, we are still getting...
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