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I have conducted several western blot experiments to detect the SUMO-conjugates bands (or smear) in tobacco total proteins extract expressing the SUMO. But, I could not detect the SUMO-conjugated...
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I am currently conducting a Western blot analysis on A. evenia and tobacco (Nb) plant total protein extracts expressing SUMO protein in order to detect SUMOylation using an anti-SUMO antibody....
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