Fawzi Khoder-Agha

3 Questions 5 Answers 0 Followers

Questions related from Fawzi Khoder-Agha

Blue native gel fluorescence?

Hi, Did anyone visualize fluorescent bands for fluorescent tagged proteins on a blue native gel? Do you destain the gel after the run? If anyone has a working protocol I would appreciate...

12 December 2018 7,467 5 View

Site directed mutagenesis of 5 consecutive sites in one reaction?

Hi, I am willing to mutate 5 specific consecutive amino acids to Alanine in a certain insert using site-directed mutagenesis in one PCR reaction only. I'm scanning a certain area of my protein to...

01 January 2018 3,841 1 View

Crowding and false FRET problem?

I'm using the Operetta system to measure the FRET signal between two proteins in the Golgi (tags are YFP and mCherry). The majority of the signal comes from protein crowding and not from real...

08 August 2017 9,221 5 View