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Questions related from Fawzi Khoder-Agha
Hi, Did anyone visualize fluorescent bands for fluorescent tagged proteins on a blue native gel? Do you destain the gel after the run? If anyone has a working protocol I would appreciate...
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Hi, I am willing to mutate 5 specific consecutive amino acids to Alanine in a certain insert using site-directed mutagenesis in one PCR reaction only. I'm scanning a certain area of my protein to...
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I'm using the Operetta system to measure the FRET signal between two proteins in the Golgi (tags are YFP and mCherry). The majority of the signal comes from protein crowding and not from real...
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