Fawad ali Shah

4 Questions 11 Answers 0 Followers

Questions related from Fawad ali Shah

Any advice on the problem in counterstaining with methyl green and cytoplasmic protein identification?

For nuclei staining, i washed my DAB stain slides with either PBS/dH2O and add few drops of methyl green, but it did not work even if i stay for 60 mints. If somehow some slight counter stain is...

11 November 2015 2,244 2 View

Which area of cortex specifically i should focus for collecting sample after MCAo?

I hope you all will be doing well. I have a question regarding cortical sample collection after MCAo surgery. I have operated 48hrs Permanent MCAo in rats. But after collecting the samples some of...

05 May 2015 5,700 4 View

Rats die after middle cerebral artery occlusion (MCAO)?

I have a question regrading permanent MCAO. I am using rats for my experimental model by means of 4-0 nylon with rounded tip (heated manually) and I coat the nylon with poly lysine before...

10 October 2013 2,634 16 View

Is anyone working on the 2 Dimensional gel electrophoresis (2D) USING tissue sample?

Hi every body. If someone is working on the 2 Dimensional gel electrophoresis (2D) USING tissue sample, i have some question regarding focusing step. the focusing result of pH 4-7 give clear spots...

09 September 2013 8,146 7 View