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I am working on a non-model plant. The sequence of my organism does not exist. I have a RT PCR product that i amplified with primers of the plants within same family. The length of my amplicons...
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Hello, In my RT-PCR results, I got cq values more about 30 for reference gene Tubulin. But Cq values of gene of interest are 25. What should i do?
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We want to discover SNPs in our breeding materials. Cooperation of KASPar and Fluidigm is succesful to identify SNPs, but if we dont know SNPs how we can discover through Kaspar system?...
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