Have you ever counted lymphocutes (absolute numbers) using Accuri C6 cytometer from BD biosciences?

We stained 50mcl of whole human blood with anti-CD3 (SK7 clone, APC, BD biosciences). After 30 min of staining we added 1ml of x1 RBC buffer (42240, Biolegend) and incubated 15 min. The procedure...

26 October 2017 1,445 1 View