Fatima Carla Bertulfo

2 Questions 2 Answers 0 Followers

Questions related from Fatima Carla Bertulfo

When performing a dual luciferase reporter assay, is it recommended to have the same promoter for the expression vector and renilla plasmid?

My transgene is in a pcDNA3 vector with a CMV promoter while my Renilla internal control vector has an HSV-TK promoter. I'm just wondering if I should use a Renilla vector with the same promoter...

05 November 2017 2,569 1 View

5 different sets of qPCR primers do not amplify gene of interest in a particular cell line?

Hi,I've designed qPCR primers to target my gene of interest in HeLa cell line. I designed them such that it will encompass an exon-exon junction and it the product is 100-200bp long. Apparently,...

26 September 2017 4,848 3 View