Farwa Naqvi

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Questions related from Farwa Naqvi

Fixed Human brain sections on slide. The thickness of the brain section is 50 μm. How to get rid of the background and how to get good staining ?

Using formalin fixed brain sections. Sections are 50μm thick and fixed on the slide. Was using 1 hour antigen retrieval in Citrate Buffer (ready made, sigma one) at 90C temperature. Then...

30 November 2020 6,722 4 View

What should be the percentage of Triton-x100 and Tween-20 in staining formalin fixed Human brain tissues?

Hi, Which percentage of Triton x100 and Tween-20 is best for staining formalin fixed human brain tissues? 0.1%, 0.5% or 1%? Which blocking agent could be used and in which percentage? I want good...

18 October 2020 7,712 4 View