Faruk Abdullah Saurav

3 Questions 3 Answers 0 Followers

Questions related from Faruk Abdullah Saurav

What can be the reason for CD63 and CD81 were detected by western blot but not by ms analysis.?

Hi, I isolated exosome to perform proteomic analysis by PEG precipitation method, I can see exosome like vesicles in SEM/TEM with in the size range, I have done western blot with CD63, 81, 9,...

11 November 2017 2,164 4 View

How can you improve your 2D gel?

I am trying to run 2D gel. I am extracting protein from mammalian cell by using RIPA lysis buffer and then precipitate the protein with acetonitrile, then re suspend the protein in 250μl of...

02 February 2016 8,177 2 View

Can someone please tell me how to fix exosome sample on glass cover slips?

I am new to exosome research and I need to do SEM of my exosome sample. I want to fix my exosomes on the glass cover slips, every time I try to fix them, after fixation when I try to take them...

04 April 2015 5,466 4 View