Farruk Lutful Kabir

4 Questions 15 Answers 0 Followers

Questions related from Farruk Lutful Kabir

Can I store aqueous phase when extracting RNA from cell culture using Trizol/Qiazol reagents?

I am extracting RNA from cell culture/tissues using Trizol/Guanidinium thiocyanate and Phenol-chloroform method. For some reason I need to store my aqueous phase and run the rest extraction, cDNA...

20 September 2015 1,707 4 View

What is the threshold value to manifest that a gene is up-/down-regulated compared to normal/untreated sample in quantitative PCR assays?

When fold-change in expression is calculated for a sample compared to control (e.g. normal/untreated sample), if the value is exactly 1, there is no change in expression in the tested sample. But...

25 September 2014 6,308 5 View

What would be the optimum ratio of two reporter expression vectors (for gene of interest) when co-transfected in mammalian cells?

I am working with miRNA expression in canine cancer cell lines. This particular question refers to my reporter assays to test the miRNA and 3'-UTR target sequence binding in cancer cells. I am...

17 August 2014 2,195 5 View

QPCR data analysis: what is the correct way to represent the results?

I am doing qPCR using SYBR Green fluorescent dye. I am looking at gene expression of some biomarkers in cancer cells. I have GAPDH or L37 as reference gene and also a normal cell line related to...

07 August 2014 1,385 3 View