Farhat A. Avin

5 Questions 14 Answers 0 Followers

Questions related from Farhat A. Avin

What are the controversies surrounding DNA barcoding of fungi?

I am gathering different opinions regarding to DNA barcoding of fungi. Please enumerate the major obstacles we are currently facing. And if there is any recommendation?

08 August 2017 8,815 2 View

Do we need to have replications for PCR-RFLP?

I am going to use PCR-RFLP method for identification purpose. Is it necessary to have replications in the experience? Either in different clones or in amplification or in digestion?

06 June 2014 2,544 10 View

Can anyone help with restriction digestion time optimization?

Does anyone know how long should be sufficient for digestion of a 4kb pcr fragment? in the manual mentioned 1-16 hours, but I don't have sufficient product to optimize all possible hours.

03 March 2014 3,442 12 View

Is there any referenced sequence for laccase gene of Pleurotus spp?

I'm looking for the full sequence of laccase gene in Pleurotus spp.

03 March 2014 6,284 2 View

Can anyone help me to solve the problem described below?

Enclosed is the estimation of covariance for a 7x7 diallel cross design that I found in a workshop manual. However, using the formula and example provided, I am not able to calculate w2, w3,...

02 February 2013 7,510 10 View